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hybridization solution (50 μl)  (Agilent technologies)


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    Structured Review

    Agilent technologies hybridization solution (50 μl)
    Hybridization Solution (50 μl), supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hybridization+solution+(50+%CE%BCl)/pmc06697070-133-0-10
    Average 90 stars, based on 1 article reviews
    hybridization solution (50 μl) - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Hybridization:

    Article Title: Transcriptomic analysis of the bitter taste receptor-mediated glucagon-like peptide-1 stimulation effect of quinine
    Article Snippet: Quinine is a bitter taste receptor agonist that has been studied its anti-pyretic, anti-malarial, antipain, and anti-inflammatory activity.. In this study, glucagon-like peptide-1 (GLP-1) stimulation effect of quinine was investigated.. Bitter taste receptors are G protein-coupled receptor (GPCR), which transfer the molecular signal through its downstream cascade.

    Article Title: Role of IL-1ra and Granzyme B as biomarkers in active Crohn’s disease patients
    Article Snippet: Background and Aim: Altered gene expression in intestinal mucosa is thought to contribute to inflammatory process in Crohn’s disease (CD).. The present study investigated changes in the expression of genes associated with gut inflammation in CD patients by RNA microarray to identify disease biomarkers.. Methods: Microarray analysis was carried out in formalin-fixed, paraffin-embedded intestinal tissue specimens from six CD patients who underwent surgery without prior treatment and from two healthy control subjects.

    Article Title: The p53 activator overcomes resistance to ALK inhibitors by regulating p53-target selectivity in ALK-driven neuroblastomas
    Article Snippet: Finally, 2× GE hybridisation buffer (25 μL) was added to dilute the labelled cRNA. .. Hybridisation solution (50 μL) was dispensed into the gasket slide and placed on the SurePrint G3 Human Microarray, 8×60 K (Agilent Technologies). .. The slides were then incubated for 17 h at 65 °C in an Agilent hybridisation oven, following which they were washed at room temperature according to the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology, v6.5, 2010).

    Article Title: Innate immune response gene expression profiles in specific pathogen-free chickens infected with avian influenza virus subtype H9N2
    Article Snippet: In poultry species, H9N2 low pathogenic avian influenza (LPAI) virus infections frequently lead to low to moderate mortality rates and morbidity characterized by depression, respiratory disease symptoms, and decreases in egg production.. Since current knowledge on the avian immune response to H9N2 infection is limited, we used microarray analysis to examine global changes in immune-related gene expression induced by H9N2 LPAI infection in specific pathogenfree chickens.. The expression profiles of approximately 800 genes, including those that influence cell differentiation, transcription, transport, immune responses, and signal transduction, were altered by H9N2 infection.

    Article Title: Aqueous extracts of Anemarrhena asphodeloides stimulate glucagon-like pepetide-1 secretion in enteroendocrine NCI-H716 cells
    Article Snippet: Anemarrhena asphodeloides (AA), a bitter taste herbal medicine, has been prescribed in traditional oriental medicine to treat diabetes mellitus.. Here, AA was extracted and fractionated to investigate its effects on the stimulation of glucagon-like peptide-1 (GLP1) secretion in enteroendocrine cells.. GLP-1 is secreted from the human enteroendocrine L cells to the blood in response to ingested nutrients.

    Article Title: Genomic analysis of lncRNA and mRNA profiles in circulating exosomes of patients with rheumatic heart disease
    Article Snippet: .. 50 μl of hybridization solution was assembled to the lncRNA expression microarray slide and incubated for 17 h at 65°C in an Agilent Hybridization Oven. .. The hybridized arrays were washed, fixed and scanned using the Agilent DNA Microarray Scanner (part number G2505C).

    Article Title: Kr?ppel-like Factor 4 (KLF4) Activates the Transcription of the Gene for the Platelet Isoform of Phosphofructokinase (PFKP) in Breast Cancer
    Article Snippet: Microarray Analysis Each total RNA (200 ng) from KLF4 knockdown or control MCF7 cells was labeled and amplified using the Low Input Quick Amp labeling kit (Agilent Technologies, Santa Clara, CA). .. The Cy3-labeled aRNAs were resuspended in 50 μl of hybridization solution (Agilent technologies). .. After labeled aRNAs were placed on Agilent SurePrint G3 Human GE 8 × 60K array (Agilent technologies) and covered by a Gasket 8-plex slide (Agilent Technologies).

    Article Title: Differential Expression Profiles and Functional Prediction of Circular RNAs and Long Non-coding RNAs in the Hippocampus of Nrf2-Knockout Mice
    Article Snippet: Finally, 25 μl 2× hybridization buffer (Agilent, Cat No. 5190-0403, United States) was added to dilute the labeled cRNA. .. Hybridization solution (50 μl) was dispensed into the gasket slide (Agilent, Cat No. G2534-60003, United States) and assembled to a mouse circRNA microarray (8 × 15K, Arraystar) slide. .. In an Agilent hybridization oven (Agilent, Cat No. G2545A, United States), the slides were incubated for 17 h at 65°C.

    Microarray:

    Article Title: The p53 activator overcomes resistance to ALK inhibitors by regulating p53-target selectivity in ALK-driven neuroblastomas
    Article Snippet: Finally, 2× GE hybridisation buffer (25 μL) was added to dilute the labelled cRNA. .. Hybridisation solution (50 μL) was dispensed into the gasket slide and placed on the SurePrint G3 Human Microarray, 8×60 K (Agilent Technologies). .. The slides were then incubated for 17 h at 65 °C in an Agilent hybridisation oven, following which they were washed at room temperature according to the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology, v6.5, 2010).

    Article Title: Genomic analysis of lncRNA and mRNA profiles in circulating exosomes of patients with rheumatic heart disease
    Article Snippet: .. 50 μl of hybridization solution was assembled to the lncRNA expression microarray slide and incubated for 17 h at 65°C in an Agilent Hybridization Oven. .. The hybridized arrays were washed, fixed and scanned using the Agilent DNA Microarray Scanner (part number G2505C).

    Article Title: Differential Expression Profiles and Functional Prediction of Circular RNAs and Long Non-coding RNAs in the Hippocampus of Nrf2-Knockout Mice
    Article Snippet: Finally, 25 μl 2× hybridization buffer (Agilent, Cat No. 5190-0403, United States) was added to dilute the labeled cRNA. .. Hybridization solution (50 μl) was dispensed into the gasket slide (Agilent, Cat No. G2534-60003, United States) and assembled to a mouse circRNA microarray (8 × 15K, Arraystar) slide. .. In an Agilent hybridization oven (Agilent, Cat No. G2545A, United States), the slides were incubated for 17 h at 65°C.

    Expressing:

    Article Title: Genomic analysis of lncRNA and mRNA profiles in circulating exosomes of patients with rheumatic heart disease
    Article Snippet: .. 50 μl of hybridization solution was assembled to the lncRNA expression microarray slide and incubated for 17 h at 65°C in an Agilent Hybridization Oven. .. The hybridized arrays were washed, fixed and scanned using the Agilent DNA Microarray Scanner (part number G2505C).

    Incubation:

    Article Title: Genomic analysis of lncRNA and mRNA profiles in circulating exosomes of patients with rheumatic heart disease
    Article Snippet: .. 50 μl of hybridization solution was assembled to the lncRNA expression microarray slide and incubated for 17 h at 65°C in an Agilent Hybridization Oven. .. The hybridized arrays were washed, fixed and scanned using the Agilent DNA Microarray Scanner (part number G2505C).



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